Loading...
Development of optimized conditions for Glaser-Hay bioconjugations
Nimmo, Zachary M. Halonski, John F. Chatkewitz, Lindsay E. Young, Douglas D.
Nimmo, Zachary M.
Halonski, John F.
Chatkewitz, Lindsay E.
Young, Douglas D.
Abstract
The efficient preparation of protein bioconjugates represents a route to novel materials, diagnostics, and therapeutics. We previously reported a novel bioorthogonal Glaser-Hay reaction for the preparation of covalent linkages between proteins and a reaction partner; however, deleterious protein degradation was observed under extended reaction conditions. Herein, we describe the systematic optimization of the reaction to increase coupling efficiency and decrease protein degradation. Two optimized conditions were identified varying either the pH of the reaction or the bidentate ligand employed, allowing for more rapid conjugations and/or less protein oxidation. (C) 2017 Elsevier Inc. All rights reserved.
Description
Date
2018-02-02
Journal Title
Journal ISSN
Volume Title
Publisher
Collections
Download Dataset
Files
Loading...
development.pdf
Adobe PDF, 720.29 KB
Rights Holder
Usage License
Embargo
Research Projects
Organizational Units
Journal Issue
Keywords
Citation
Advisor
Department
Chemistry
DOI
https://doi.org/10.1016/j.bioorg.2017.11.020