Date Thesis Awarded
Honors Thesis -- Access Restricted On-Campus Only
Bachelors of Science (BS)
Margaret S. Saha
Randolph A. Coleman
Mark H. Forsyth
The establishment of the anterior-posterior (AP) axis is an essential step in the development of the central nervous system. In the model vertebrate organism Xenopus laevis, AP neural patterning begins during the late blastula stage and continues through gastrulation. Although the patterning of the nervous system in normal conditions has been extensively studied, less is known about how this process is able to regulate in the face of environmental perturbations. This study aims to characterize the extent and molecular basis of neural axis plasticity in Xenopus laevis by investigating the response of embryos to a 180-degree rotation of their AP neural axis during gastrulation. Embryos were assessed for the expression of regional marker genes using in situ hybridization, and also underwent global gene expression analysis using RNA-Sequencing. Our results suggest that there is a window of time between the mid- and late-gastrula stage during which embryos are able to recover from a 180-degree rotation of their neural axis and then lose this ability. At the mid-gastrula stage, embryos are able to recover from neural axis rotation and correctly express regional marker genes. By the late-gastrula stage, embryos show misregulation of regional marker genes following neural axis rotation and differential expression of genes important for neural development and patterning. Heterochronic transplants between donor and host embryos of different stages indicate that both the presumptive neural ectoderm and the underlying mesoderm play an important role in this plasticity.
Bolkhovitinov, Lyuba, "Anterior-Posterior Axis Plasticity in the Developing Nervous System of Xenopus laevis" (2017). Undergraduate Honors Theses. William & Mary. Paper 1076.
On-Campus Access Only