Date Thesis Awarded

5-2020

Document Type

Honors Thesis

Degree Name

Bachelors of Science (BS)

Department

Chemistry

Advisor

Douglas Young

Committee Members

Robert Hinkle

Rachel O'Brien

Mark Forsyth

Abstract

Unnatural amino acid technologies have been critical in introducing unique chemical functionality to proteins to allow for specific protein modulation or preparation of well-defined, homogeneous bioconjugates through bioconjugation chemistry. Bioconjugates are tremendously useful in disease treatment, diagnosis, and imaging. Therefore, the development and optimization of novel unnatural amino acids and bioconjugation methods, and their application to protein modulation or bioconjugate formation is a critical field of biochemical investigation. This thesis describes the development of a [2+2+2] cyclotrimerization bioconjugation using a novel unnatural amino acid. Then, several methods of preparing multivalent conjugates, which have a broader range of therapeutic applications than typical bivalent conjugates, are discussed. Finally, efforts to assay and modulate human protein arginine methyltransferase 1 using photoregulation unnatural amino acid technology are described. This thesis aims to expand the bioconjugation chemical toolbox, with high impact medicinal, therapeutic, and material applications.

Available for download on Sunday, May 08, 2022

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