Date Thesis Awarded

5-2021

Access Type

Honors Thesis -- Access Restricted On-Campus Only

Degree Name

Bachelors of Science (BS)

Department

Interdisciplinary Studies

Advisor

Margaret Saha

Committee Members

Mainak Patel

Junping Shi

Dana Willner

Abstract

The Mycobacterium genus is responsible for 1.5-2 million deaths per year worldwide, with mortality and morbidity numbers growing as antibiotic resistance increases. M. smegmatis, a common non-pathogenic soil bacterium of this genus, serves as a drug screening platform and potential vaccine candidate for other pathogenic members. Unique metabolic properties of M. smegmatis have also rendered it an exceptional host for biomanufacturing purposes, which can be further advanced upon incorporating synthetic biology approaches. To achieve fine-tuning of synthetic circuits, a comprehensive and well-characterized library of regulatory sequences, especially promoters, is indispensable. This study aims to develop a library of M. smegmatis promoters through genome screening. We have constructed a genome analysis DataFrame by integrating genomic and transcriptomic data; a list of potential promoter regions was generated from the DataFrame. We have also experimentally characterized six M. smegmatis promoters (including a previously unidentified endogenous promoter) and standardized a cloning & characterization protocol to examine promoter function. We anticipate that these efforts contribute to the advancement of M. smegmatis applications through synthetic biology approaches.

Available for download on Sunday, May 14, 2023

On-Campus Access Only

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