Loading...
Development and evaluation of a formulation of probiontPhaeobacter inhibens S4 for the management of vibriosis inbivalve hatcheries
Takyi, Evelyn LaPorte, Jason Sohn, Saebom Stevick, Rebecca J. Witkop, Meredith M. Giray, Cem Rowley, David C. Nelson, David R. Gomez-Chiarri, Marta
Takyi, Evelyn
LaPorte, Jason
Sohn, Saebom
Stevick, Rebecca J.
Witkop, Meredith M.
Giray, Cem
Rowley, David C.
Nelson, David R.
Gomez-Chiarri, Marta
Abstract
Larval eastern oysters (Crassostrea virginica) grown in shellfish hatcheries are suscep-tible to bacterial diseases, particularly vibriosis. Probiotics are microbes that conferhealth benefits to the host and have been identified as promising tools to manage dis-eases in aquaculture. The marine bacterium Phaeobacter inhibens S4 (S4) protects larvaleastern oysters against challenge with the bacterial pathogen Vibrio coralliilyticus RE22(RE22). A concentrated liquid formulation of probiont S4 that maintained high cell via-bility after long-term storage was developed for commercial use in shellfish hatcheries.The safety and efficacy of the formulation were tested in six different trials in twohatcheries. The S4 formulation was added to C. virginica larvae culture tanks daily at10 4 colony forming units (CFU)/mL from Day 1 post fertilisation until Day 6, 12 or 14,depending on the trial. Treatment of larvae in the hatchery with the S4 formulation didnot significantly affect the survival and growth of the larvae. Formulated probiont S4treatment in the hatchery led to a significant increase in relative percent survival (RPS)when larvae were subsequently challenged with the pathogen RE22 (105 CFU/mL) for24 h in a laboratory challenge as compared to probiotic-untreated RE22-challengedlarvae (RPS increase of 46%–74%, p < 0.05). These results suggest that this novel S4 formulation is a safe, easy-to-use and effective tool in preventing larval losses due tovibriosis in hatcheries.
Description
Date
2023
Journal Title
Journal ISSN
Volume Title
Publisher
John Wiley & Sons Ltd
Download Dataset
Rights Holder
The author
Usage License
http://creativecommons.org/licenses/by/4.0/
Embargo
Research Projects
Organizational Units
Journal Issue
Keywords
Citation
Takyi, E., LaPorte, J., Sohn, S., Stevick, R.J., Witkop, E.M. & Gregg, L.S. et al. (2023) Development and evaluation of a formulation of probiont Phaeobacter inhibens S4 for the management of vibriosis in bivalve hatcheries. Aquaculture, Fish and Fisheries, 3, 256–267. https://doi.org/10.1002/aff2.112
Advisor
Department
Natural Resources
DOI
https://doi.org/10.1002/aff2.112