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Using Unnatural Amino Acid Technologies to Immobilize and Regulate Proteins
Peairs, Emily Mae
Peairs, Emily Mae
Abstract
Bioconjugates are an important area of research with widespread applications in medicine, pharmaceuticals, and materials. Therefore, it is crucial to optimize current methodologies and develop novel ways to generate these products in an efficient and scalable manner. This thesis details the use of unnatural amino acid technologies in the generation of well-defined, functional bioconjugates. First, the mechanism and optimization of the Glaser-Hay coupling is discussed to improve current bioconjugation methods. Next, I investigated the immobilization of a hyperthermophilic enzyme to establish a methodology applicable to an expanse of useful biocatalysts. Then, the development of novel multivalent products is examined. Lastly, the reversible caging of an enzyme integral in gene regulation is reported, as is the implications of such a technology. Through this work, I aimed to build on existing methodologies and create novel paths to generate innovative, effective bioconjugates for therapeutic and industrial use.
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2020-05-01
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Chemistry