Loading...
Perkinsus sp infections and in vitro isolates from Anadara trapezia (mud arks) of Queensland, Australia
Dang, C Dungan, CF Scott, GP Reece, Kimberly S
Dang, C
Dungan, CF
Scott, GP
Reece, Kimberly S
Abstract
Perkinsus sp. protists were found infecting Anadara trapezia mud ark cockles at 6 sites in Moreton Bay, Queensland, Australia, at prevalences of 4 to 100% during 2011 as determined by surveys using Ray's fluid thioglycollate medium. Perkinsus sp. lesions were found among gill and visceral connective tissues in histological samples from several cockles, where basophilic, eccentrically vacuolated Perkinsus sp. signet ring trophozoites and proliferating, Perkinsus sp. schizont cells were documented. Two Perkinsus sp. isolates were propagated in vitro during August 2013 from gill tissues of a single infected A. trapezia cockle from Wynnum in Moreton Bay. DNA from those isolate cells amplified universally by a Perkinsus genus-specific PCR assay, and rDNA-internal transcribed spacer sequences respectively grouped them with P. olseni and P. chesapeaki in phylogenetic analyses. This is the first report of P. chesapeaki in Australia, and the first report of a P. chesapeaki in vitro isolate from an Australian mollusc host. Although P. olseni was originally described in 1981 as a pathogen of abalone in South Australia, and has subsequently been identified as a prevalent pathogen of numerous other molluscs worldwide, this is also the first report of a P. olseni-like in vitro isolate from an Australian mollusc host.
Description
Date
2015-01-01
Journal Title
Journal ISSN
Volume Title
Publisher
Collections
Download Dataset
Files
Loading...
d113p051.pdf
Adobe PDF, 955.54 KB
Rights Holder
Usage License
Embargo
Research Projects
Organizational Units
Journal Issue
Keywords
Aquatic Health Sciences Peer-Reviewed Articles, Clam Ruditapes-Philippinarum; Chesapeake Bay; Mya-Arenaria; Phylogenetic Position; Tagelus-Plebeius; Olseni Protozoa; Genus Perkinsus; Ribosomal-Rna; Decussatus L; Shell Clams
Citation
Advisor
Department
Virginia Institute of Marine Science
DOI
https://doi.org/10.3354/dao02816