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Use of immunoassays in haplosporidan life cycle studies
Burreson, Eugene M.
Burreson, Eugene M.
Abstract
The development of mitigating measures for the major oyster diseases has been hindered by our poor understanding of the life cycles of the pathogens. Evidence from epidemiological studies and transmission experiments suggests that an intermediate host is present in the life cycle of Haplosporidium species. Immunoassay is a valuable tool for identifying parasite antigen in an intermediate host, and, because of the potential for stage-specific antigens, assays incorporating polyclonal antibodies may be more effective than assays incorporating monoclonal antibodies. Rabbit antibody against purified spores of Haplosporidium costale recognized spores in paraffin sections of oyster tissue , but the antibody did not recognize plasmodia of H. costale.
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1988
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American Fisheries Society
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AFS18_burreson.pdf
Adobe PDF, 3.16 MB
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Virginia Institute of Marine Science