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Enumeration of Enterococcus sp. using a modified mE method
Rhodes, MW Kator, H
Rhodes, MW
Kator, H
Abstract
A modified mE medium (mEI) containing the chromogenic substrate indoxyl-beta-D-glucoside to detect beta-D-glucosidase activity was evaluated with respect to specificity and recovery of enterococci from environmental eaters. Extending incubation from 24 to 48 h improved enterococci recovery but 77% of the colonies classified as non-target were confirmed as enterococci. Randomly chosen enterococcal isolates from sewage, exposed in microcosms containing 0.22 mu m membrane filtered fresh or estuarine water, exhibited differences in persistence as a function of exposure treatment. Decreasing the concentration of or eliminating indoxyl-beta-D-glucoside from mE did not significantly affect recovery of purified isolates.
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1997
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Rhodes1997.pdf
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Aquatic Health Sciences Peer-Reviewed Articles
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Virginia Institute of Marine Science
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https://doi.org/10.1046/j.1365-2672.1997.00200.x