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Lost and Found: Localization of the Ubiquitin Ligase Slx5 to the Yeast Bud Neck
Esteban, Cecilia Natalia A.
Esteban, Cecilia Natalia A.
Abstract
Slx5 and Slx8 are two subunits of a SUMO-Targeted Ubiquitin Ligase (STUbL) in the budding yeast Saccharomyces cerevisiae. STUbLs are E3 ubiquitin ligases that target sumoylated substrates for ubiquitylation. Slx5 contains several SUMO-interacting motifs (SIMs) and is considered to be the targeting subunit of the Slx5∙Slx8 STUbL. In yeast, Slx5 forms SIM-dependent nuclear foci, some of which co-localize with double-stranded DNA breaks. To study how these foci form in the nucleus, we performed a high-throughput screen for temperature-sensitive mutants. In this screen, we identified an slx5 mutant that both formed nuclear foci and mislocalized to the bud neck. Slx5 localization at the bud neck resembled the localization pattern of septin proteins. Septins are a class of proteins involved in cytokinesis and cellular division and are transiently sumoylated at the G2/M phase of the cell cycle, making them potential Slx5?Slx8 ubiquitylation targets when nuclear import of Slx5 is impaired. Here we present our data and analysis of this Slx5 septin mislocalization phenotype. The findings of this study have great implications for understanding the STUbL activity in humans.
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Thesis is part of Honors ETD pilot project, 2008-2013. Migrated from Dspace in 2016.
Date
2010-05-11
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Keywords
SUMO, Ubiquitin, Yeast, Genetics, SUMO-Targeted Ubiquitin Ligase, Localization, Septin, Nucleus, Sumoylation, Ubiquitylation
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Department
Biology