Data from: A potential case of reinforcement in a facultatively sexual unicellular eukaryote

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The origin of a new species requires a mechanism to prevent divergent populations from interbreeding. In the classic allopatric model, divided populations evolve independently and accumulate genetic differences. If contact is restored, hybrids suffer reduced fitness and selection may favor traits that prevent mistakes in mating, a process known as reinforcement. This decisive but transient phase is challenging to document and has been reported mostly in macroorganisms. Very little is known about the processes through which new microbial species originate. In particular, it is unclear whether microbial eukaryotes, many of which can reproduce sexually during complex life cycles, speciate in much the same way as do well-studied plants and animals. Using individual cellular mate choice trials, we investigated the mating behavior of sympatric and allopatric woodland populations of the yeast Saccharomyces paradoxus. We find evidence consistent with reinforcement, potentially representing an example of microbial speciation in progress.,Mating DataThis file contains the results of mate choice trials in Saccharomyces paradoxus strains from three different populations/groups. Each mating trial contained two spores from one strain (the chooser strain) and one spore from another strain (the option strain). If the two spores from one strain mated, it was classified as inbreeding; if two spores from different strains mated, it was classified as outcrossing. The data are in columns with the following headers: Chooser Group; Option Group; Chooser Strain; Option Strain; Number of Outcrossing; Number of Inbreeding; Total Trials.Sp_Data_Counts.csvSpSporeViabilityThese are spore viability data for individual Saccharomyces paradoxus strains, as well as crosses between them. There are numerous columns with different identifiers (genetic group, strain, within/between population, etc.) for different analyses.GerminationTimingThis file contains data collected by monitoring the amount of time it took for spores from different Saccharomyces paradoxus strains to germinate. Each column is a different strain (headers included).,



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