Title

Seawater carbonate chemistry and shell dissolution in dead gastropod larvae and adult Limacina helicina pteropods

Document Type

Data

DOI

PANGAEA.899574

Publication Date

3-25-2019

Description

Abstract: Ocean acidification (OA) increases aragonite shell dissolution in calcifying marine organisms. It has been proposed that bacteria associated with molluscan shell surfaces in situ could damage the periostracum and reduce its protective function against shell dissolution. However, the influence of bacteria on shell dissolution under OA conditions is unknown. In this study, dissolution in dead shells from gastropod larvae and adult pteropods (Limacina helicina) was examined following a 5-day incubation under a range of aragonite saturation states (Ωarag; values ranging from 0.5 to 1.8) both with and without antibiotics. Gastropod and pteropod specimens were collected from Puget Sound, Washington (48°33′19″N, 122°59′49″W and 47°41′11″N, 122°25′23″W, respectively), preserved, stored, and then treated in August 2015. Environmental scanning electron microscopy (ESEM) was used to determine the severity and extent of dissolution, which was scored as mild, severe, or summed (mild + severe) dissolution. Shell dissolution increased with decreasing Ωarag. In gastropod larvae, there was a significant interaction between the effects of antibiotics and Ωarag on severe dissolution, indicating that microbes could mediate certain types of dissolution among shells under low Ωarag. In L. helicina, there were no significant interactions between the effects of antibiotics and Ωarag on dissolution. These findings suggest that bacteria may differentially influence the response of some groups of shelled planktonic gastropods to OA conditions. This is the first assessment of the microbial–chemical coupling of dissolution in shells of either gastropod larvae or adult L. helicina under OA. Category: geoscientificInformation Source: Supplement to: Bausch, Alexandra Renee; Gallego, M Angeles; Harianto, Januar; Thibodeau, Patricia; Bednaršek, Nina; Havenhand, Jonathan N; Klinger, Terrie (2018): Influence of bacteria on shell dissolution in dead gastropod larvae and adult Limacina helicina pteropods under ocean acidification conditions. Marine Biology, 165(2), https://doi.org/10.1007/s00227-018-3293-3 Supplemental Information: In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2019-03-25. Coverage: EVENT LABEL: * LATITUDE: 47.686390 * LONGITUDE: -122.423060 * DATE/TIME START: 2014-10-30T00:00:00 * DATE/TIME END: 2015-08-15T00:00:00 * METHOD/DEVICE: Experiment EVENT LABEL: * LATITUDE: 48.555280 * LONGITUDE: -122.996940 * DATE/TIME START: 2014-10-30T00:00:00 * DATE/TIME END: 2015-08-15T00:00:00 * METHOD/DEVICE: Experiment

Publisher

PANGAEA

Language

eng

Source Link

https://doi.pangaea.de/10.1594/PANGAEA.899574

Share

COinS