Document Type

Report

Department/Program

Virginia Institute of Marine Science

VIMS Department/Program

Marine Advisory Program (MAP)

Publication Date

2003

Series

Fishery Resource Grant FRG 2003-01

Abstract

This validation study was conducted during the months of July, August and Sept., 2003. The Food and Drug Administration and the Interstate Shellfish Sanitation Conference require that oysters used for a validation study are collected during the same processing day, are from the same lot of shellfish, and have an adjusted geometric mean (AGM) MPN of at least I 00,000/g of Vibrio vulnificus as an initial load. The oysters were removed from refrigerated storage and held at 71 °F (21 °C) for 40-45 hours prior to freezing in order to achieve a minimum AGM of 100,000/g Vibrio vulnificus . After quick freezing the oysters at -70°F (-57°C) for nine minutes, approximately 8-10 weeks of frozen storage at 0°F (-l 8°C) were required to reduce V. vulnificus levels from 105 MPN/g to less than 30MPN/g. Initial V. vulnificus numbers in excess of 1,000,000/g, required prolonged frozen storage (>20 weeks) of the oysters at 0°F (-18°C) to reduce V. vulnificus numbers to less than 30MPN/g.

Keywords

Oyster aquaculture

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