Changes in bacterioplankton metabolic capabilities along a salinity gradient in the York River estuary, Virginia, USA

PA Raymond
JE Bauer

Abstract

We measured dissolved organic carbon (DOC), dissolved inorganic carbon (DIC), and their corresponding Delta C-14 and delta C-13 values in order to study the sources and fates of DOC in the York River Estuary (Virginia, U.S.A.). The Delta C-14 and delta C-13 values of DOC and DIC at the freshwater end-member indicate that during periods of moderate to high flow, riverine DOC entering the York was composed of decadal-aged terrestrially organic matter. In nearly all cases, DOC concentrations exceeded conservative mixing lines and were therefore indicative of a net DOC input flux from within the estuary that averaged 1.2 muM L-1 d(-1). The nonconservative behavior of DOC in the York River Estuary was also apparent in carbon isotopic mixing curves and the application of an isotopic mixing model. The model predicted that 20-38% of the DOC at the mouth of the estuary was of riverine (terrestrial + freshwater) origin, while 38-56% was added internally, depending on the isotopic values assigned to the internally added DOG. Measurements of Delta C-14 and delta C-13 of DOC and DIC and marsh organic matter suggest that the internal sources originated from estuarine phytoplankton and marshes. The isotopic mixing model also indicates a significant concomitant loss (27-45%) of riverine DOC within the estuary. Changes in DOC concentration, Delta C-14-DOC, and delta C-13-DOC were also measured during incubation experiments designed to quantify the amounts, sources, and ages of DOC supporting the carbon demands of estuarine bacteria. Results of these experiments were consistent with an estuarine source of phytoplankton and marsh DOC and the preferential utilization of youngs (C-14-enriched) DOC in the low-salinity reaches of the York. However, the average removal of riverine DOC by bacteria accounts for only similar to4-19% of the riverine pool: therefore, other significant sinks for DOC exist within the estuary.