Gas chromatographic isolation of individual compounds from complex matrices for radiocarbon dating

EJ Heist
JA Musick
JE Graves

Abstract

The Atlantic sharpnose shark, Rhizoprionodon terraenovae, is a small coastal shark that is harvested in both directed and nondirected fisheries throughout its range. Because pups of this species are found both along the southeastern U.S. Atlantic coast and the Gulf of Mexico, it is possible that multiple isolated breeding stocks exist. Restriction fragment length polymorphism analysis of mitochondrial DNA was used to test the hypothesis that Atlantic sharpnose sharks from the U.S. Atlantic coast and the western Gulf of Mexico have identical mitochondrial haplotype frequencies and therefore no apparent genetic stock structure. Seven mitochondrial haplotypes were detected among 52 individuals. The distribution of haplotypes between samples did not differ significantly from homogeneity (P=0.694), indicating that the null hypothesis of a single breeding population could not be rejected.