The In-vitro life-cycle of a Perkinsus species (Apicomplexa, Perkinsidae) isolated from Macoma-Balthica (Linneaus, 1758)

SJ Kleinschuster
FO Perkins, Virginia Institute of Marine Science
MJ Dykstra
SL Swink

Abstract

Using standard sterile techniques and a single medium previously described (Kleinschuster and Swink 1993), the in vitro culture of a Perkinsus species isolated from Macoma balthica was possible. Zoosporulation, the release of zoospores, and the reestablishment of secondary cultures from the zoospores completed an in vitro life cycle.