Virginia Institute of Marine Science
Journal Of Shellfish Research
A molecular genetic identification key for nine species of Crassostrea oysters was developed based on restriction fragment-length polymorphism (RFLP) analyses of nuclear and mitochondrial DNA markers. Seven of nine species were unambiguosly differentiated based on digestion of the ITS-1 nuclear marker with Hae III an Hinf I. Individual species exhibited one or two RFLP patterns for each restriction endonuclease, and only C. madrasensis and C. iredalei were indistinguishable electrophoretically. All nine species were unambiguosly differentiated based on digestion of the COI mitochondrial marker with Dde I and Hae III. Species exhibited one or two RFLP patterns for each restriction endonuclease, and species pairs unresolved by the first restriction enzyme were completely resolved with the second. The resulting key distinguishes among many Indo-Pacific Crassostrea oysters that overlap across some or all on their ranges, and establish as an expandable framework for future additions of other species to the key.
Crassostrea Oysters; Species Identification; Its-1; Coi; Pcr/Rflp
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Cordes, Jan F.; Xiao, Jie; and Reece, Kimberly S., Discrimination Of Nine Crassostrea Oyster Species Based Upon Restriction Fragment-Length Polymorphism Analysis Of Nuclear And Mitochondrial Dna Markers (2008). Journal Of Shellfish Research, 27(5), 1155-1161.