Virginia Institute of Marine Science
Journal of Shellfish Research
This paper describes a radiometric technique developed to measure phagocytosis of Perkinsus marinus zoospores by oyster hemocytes. The spores of P. marinus were radiolabeled by culturing P. marinus presporangia and sporangia in estuarine water (22%o) containing 14C-glycine. The percent of spores phagocytized by hemocytes was determined by the uptake of radioactivity by hemocytes.
Results from preliminary experiments to test the efficiency of using an osmotic infiltration method for immunizing oysters are also reported. It was found that oysters can take up both dissolved antigen (radiolabeled bovine serum albumin) and particulate antigen ( 14C-labeled zoospore homogenate) through osmotic infiltration. The uptake of the antigen was correlated with the concentration of antigen added to the water but was not affected by water temperature
Oyster, acquired immunity, techniques.
Chu, Fu-lin, Development and evaluation of techniques to study acquired immunity to Perkinsus marinus in the oyster, Crassostrea virginica (Gmelin) (1988). Journal of Shellfish Research, 7(1), 51-55.