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Document Type

Book Chapter


Virginia Institute of Marine Science


Joseph A. Mihursky and Ann Chaney

Publication Date


Book Title

New Perspectives in the Chesapeake System: A Research and Management Partnership, Proceedings of a Conference December 4-6, 1990 Baltimore, MD


Chesapeake Research Consortium


CRC Publication No. 137


The liver is an important site of metabolism and effects of toxic chemicals. This makes it an important organ for tumorigenesis studies. This study was aimed at investigating the optimal culture characteristics of liver cells derived from some indigenous Chesapeake Bay fish species. Hepatocytes from Atlantic -menhaden (Brevootia tyraMus), toadfish (Opsanus tau), croaker (Micropogonius undulatus), and hogchocker (Trinectes maculatus) were isolated using an enzymatic dispersion technique. The highest yield was obtained from toadfish livers (14.9±5. lx 1 o5 cells/g oflive weight) and the lowest was from the croaker (5.9±3. lx 1 o5 cells/g of live body weight). The percentage of hepatocytes in cell preparations exceeded 90% except for the toadfish liver cells preparation (82.3%). Freshly isolated hepatocytes were globular with irregular contour and occurred as individual cells or small clusters of 2-30 cells. A confluent monolayer of attached cells was obtained after two weeks of incubation. An osmolality of 325 m Osm/kg best supported the multiplication of hepatocytes of the four fish species. While the optimum temperature for hogchocker and toadfish hepatocytes appeared to be 33oC; it ranged from 21-27oC for menhaden and croaker. The medium that best supported the growth of culture

The potential of long term hepatocyte culture using fish of Chesapeake Bay will enable further studies on the activation and detoxification processes of xenobiotics and, therefore, help in understanding pollution related neoplasia and diseases

Long Term Culture of Hepatocytes of Some Fish Species of the Chesapeake Bay