Date Awarded

2019

Document Type

Thesis

Degree Name

Master of Science (M.Sc.)

Department

Chemistry

Advisor

Randolph A Coleman

Committee Member

Lisa M Landino

Committee Member

Nathanael M Kidwell

Committee Member

Frank J Castora

Abstract

Alzheimer’s disease is a specific form of dementia characterized by the aggregation of Amyloid-β plaques and tau tangles. New research has found that the formation of these aggregates occurs after dysregulation of respiratory activity and the production of radical oxygen species. Proteomic data shows that these changes are also related to unique gene expression patterns. We investigate the impact of these findings on new therapeutic options via metabolic flux analysis of sirtuin stress response pathways and respiratory supercomplex formation. Our results indicate CRISPR Cas-based gene therapy focused on upregulating stable CIII expression, and protective changes in SIRT1 and AMPK expression are potential avenues for therapeutics. This work also highlights the importance of metabolic enzyme activity in maintaining proper respiratory activity.

DOI

http://dx.doi.org/10.21220/s2-c9zh-am42

Rights

© The Author

Available for download on Sunday, June 07, 2020

Included in

Biochemistry Commons

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