Date Awarded
Winter 2015
Document Type
Dissertation
Degree Name
Doctor of Philosophy (Ph.D.)
Department
Applied Science
Advisor
Robert Vold
Abstract
The purpose of devising and validating models for intramolecular motions for FMOC amino acids is to quantify side chain motion in proteins which plays an important role in understanding biological structure function relations of proteins. In this thesis, spin lattice relaxation times (Ti) of FMOC amino acids were m easured under both static and magic angle spinning (MAS) condition at variable tem peratures. Lower activation energies of the relaxation times than the normal amino acids observed indicate a less sterically crowded environment for the rotation methyl group. A three-site jump model for the methyl group w as developed to fit the Tiz and Tiq anisotropy under static condition. Under MAS, Multiple deuterated sites can be resolved and studied independently. Finally, a tem perature model for the spinning rotor w as developed to account for the tem perature gradient across the rotor. A com parison of using the single most probable tem perature and the tem perature distribution in the simulation of relaxation times concludes the difference between th ese two approaches is minimal.
DOI
https://doi.org/10.21220/3fhf-xz16
Rights
© The Author
Recommended Citation
Xu, Wei, "Studies of molecular dynamics of FMOC Amino Acids using solid state deuteron nuclear magnetic resonance spectroscopy" (2015). Dissertations, Theses, and Masters Projects. William & Mary. Paper 1563899059.
https://doi.org/10.21220/3fhf-xz16
