Date Thesis Awarded

5-2018

Document Type

Honors Thesis

Degree Name

Bachelors of Science (BS)

Department

Kinesiology & Health Sciences

Advisor

Michael Brennan Harris

Committee Members

Helen A. Murphy

Evie Burnet

Abstract

Regular endurance exercise improves endothelium-dependent vasorelaxation and results in minimized cardiovascular disease (CVD) risk. The effect of acute exercise on the other hand, is more unclear. It has been proposed that the initial stress of acute exercise may impair vascular function prior to the onset of a beneficial adaptive response. In this study, we examined the time course of impairment in endothelial function in rat aortas following an acute, exhaustive bout of treadmill exercise. Along with that, we examined the corresponding magnitude of observed impairment and eNOS expression, SIRT1 expression, and antioxidant capacity (mM). We used 7-9-month-old, male, Wistar rats which were divided up into four groups (n=12/group): sedentary (SED), 6h post-exercise (6h), 24h post-exercise (24h) and 48h post-exercise. Exercise consisted of one bout of exhaustive treadmill exercise lasting between 30-40min. eNOS and SIRT1 expression was determined by Western Blot, antioxidant capacity (mM) was determined by performing a Caymans chemicals assay and endothelium-dependent vasorelaxation was assessed by constructing an acetylcholine dose response curve (10-9-10-5 M) in a wire myograph. eNOS expression demonstrated a significant (p

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