Document Type
Article
Department/Program
General Education
Journal Title
PLoS One
Pub Date
10-15-2015
Editor
Anita B. Hjelmeland
Place of Publication
San Francisco, California, US
Volume
10
Issue
10
Journal Article URL
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4607481/
Abstract
Translocation to the nucleus of diacylglycerol kinase (DGK)– ζ is dependent on a sequence homologous to the effector domain of Myristoylated Alanine Rich C-Kinase Substrate (MARCKS). These data would suggest that MARCKS could also localize to the nucleus. A single report demonstrated immunofluorescence staining of MARCKS in the nucleus; however, further experimental evidence confirming the specific domain responsible for this localization has not been reported. Here, we report that MARCKS is present in the nucleus in GBM cell lines. We then over-expressed wild-type MARCKS (WT) and MARCKS with the effector domain deleted (ΔED), both tagged with V5-epitope in a GBM cell line with low endogenous MARCKS expression (U87). We found that MARCKS-WT localized to the nucleus, while the MARCKS construct without the effector domain remained in the cytoplasm. We also found that over-expression of MARCKS-WT resulted in a significant increase in total cellular phosphatidyl-inositol (4,5) bisphosphate (PIP2) levels, consistent with prior evidence that MARCKS can regulate PIP2 levels. We also found increased staining for PIP2 in the nucleus with MARCKS-WT over-expression compared to MARCKS ΔED by immunofluorescence. Interestingly, we observed MARCKS and PIP2 co-localization in the nucleus. Lastly, we found changes in gene expression when MARCKS was not present in the nucleus (MARCKS ΔED). These data indicate that the MARCKS effector domain can function as a nuclear localization signal and that this sequence is critical for the ability of MARCKS to regulate PIP2 levels, nuclear localization, and gene expression. These data suggests a novel role for MARCKS in regulating nuclear functions such as gene expression.
Recommended Citation
Rohrbach, T. D., Shah, N., Jackson, W. P., Feeney, E. V., Scanlon, S., Gish, R., Khodadadi, R., Hyde, S. O., Hicks, P. H., Anderson, J. C., Jarboe, J. S., & Willey, C. D. (2015). The Effector Domain of MARCKS Is a Nuclear Localization Signal that Regulates Cellular PIP2 Levels and Nuclear PIP2 Localization. PloS one, 10(10), e0140870. https://doi.org/10.1371/journal.pone.0140870
DOI
https://doi.org/10.1371/journal.pone.0140870
Publisher Statement
PLoS One. 2015; 10(10): e0140870. Published online 2015 Oct 15. doi: 10.1371/journal.pone.0140870
