Document Type
Report
Department/Program
Virginia Institute of Marine Science
VIMS Department/Program
Marine Advisory Program (MAP)
Publication Date
2003
Series
Fishery Resource Grant FRG 2003-01
Abstract
This validation study was conducted during the months of July, August and Sept., 2003. The Food and Drug Administration and the Interstate Shellfish Sanitation Conference require that oysters used for a validation study are collected during the same processing day, are from the same lot of shellfish, and have an adjusted geometric mean (AGM) MPN of at least I 00,000/g of Vibrio vulnificus as an initial load. The oysters were removed from refrigerated storage and held at 71 °F (21 °C) for 40-45 hours prior to freezing in order to achieve a minimum AGM of 100,000/g Vibrio vulnificus . After quick freezing the oysters at -70°F (-57°C) for nine minutes, approximately 8-10 weeks of frozen storage at 0°F (-l 8°C) were required to reduce V. vulnificus levels from 105 MPN/g to less than 30MPN/g. Initial V. vulnificus numbers in excess of 1,000,000/g, required prolonged frozen storage (>20 weeks) of the oysters at 0°F (-18°C) to reduce V. vulnificus numbers to less than 30MPN/g.
Keywords
Oyster aquaculture
Recommended Citation
Bevans, R., & Cowart, L. (2003) Validation of an Individual Quick Freezing (IQF) Process to Reduce Vibrio vulnificus Numbers to <30MPN/g in Raw Oysters. Fishery Resource Grant FRG 2003-01. Virginia Institute of Marine Science, William & Mary. https://scholarworks.wm.edu/reports/2237
