Virginia Institute of Marine Science
Limnology and Oceanography
Laser fluorosensing and epifluorescence microscopy were used jointly to identify the origin of different spectral peaks of phycoerythrin in estuarine and coastal samples. The fluorescence of the samples was also examined as a function of the time elapsed after a water circulation system was turned on. Coastal samples were dominated by cyanobacteria and exhibited a constant phycoerythrin fluorescence with time. The phycoerythrin fluorescence of the Chesapeake Bay estuarine samples first increased strongly, reached a maximum, and then decreased to below the original level; these samples were dominated by cryptophytes which epifluorescence techniques revealed were being destroyed by the circulation system. A simple mathematical model was developed to describe the effects of cell disruption, the uncoupling of energy transfer between pigments, and the subsequent breakdown of the solubilized phycoerythrin.
Exton, RJ; Houghton, WM; Esaias, W; Haas, LW; and Hayward, D, Spectral differences and temporal stability of phycoerythrin fluorescence in estuarine and coastal waters due to the domination of labile cryptophytes and stabile cyanobacteria (1983). Limnology and Oceanography, 28(6), 1225-1231.