Date Thesis Awarded
5-2022
Access Type
Honors Thesis -- Access Restricted On-Campus Only
Degree Name
Bachelors of Science (BS)
Department
Neuroscience
Advisor
Christopher A. Del Negro
Committee Members
Margaret S. Saha
M. Drew LaMar
Gregory D. Conradi Smith
Abstract
Neurons in the brainstem preBötzinger complex (preBötC) generate the rhythm and rudimentary motor pattern for inspiratory breathing movements. We performed whole-cell patch-clamp recordings from inspiratory neurons in the preBötC of neonatal mouse slices that retain breathing-related rhythmicity in vitro. We classified neurons based on their electrophysiological properties and genetic background, and then aspirated their cellular contents for single-cell RNA sequencing (scRNA-seq). This data set provides the raw nucleotide sequences (FASTQ files) and annotated files of nucleotide sequences mapped to the mouse genome (mm10 from Ensembl), which includes the fragment counts, gene lengths, and fragments per kilobase of transcript per million mapped reads (FPKM). These data reflect the transcriptomes of the neurons that generate the rhythm and pattern for inspiratory breathing movements.
Recommended Citation
David, Caroline K., "Nucleotide Sequences and Transcriptomes of Inspiratory Neurons of the PreBötzinger Complex in Neonatal Mice" (2022). Undergraduate Honors Theses. William & Mary. Paper 2057.
https://scholarworks.wm.edu/honorstheses/2057
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