Document Type

Article

Department/Program

Virginia Institute of Marine Science

Publication Date

1989

Journal

Cytometry

Volume

10

Issue

5

First Page

551

Last Page

557

Abstract

This paper describes an algorithm for calculating the biovolume of cells with simple shapes, such as bacteria, flagellates, and simple ciliates, from a 2-dimensional digital image. The method can be adapted to any image analysis system which allows access to the binary cell image-( i.e., the pixels, or (x,y) points, composing the cell. The cell image is rotated to a standard orientation (horizontal), inand a solid of revolution is calculated by digital integration. Verification and a critical assessment of the method are presented. The algorithm accounts for irregularities in cell shape that conventional methods based on length, width, and geometrical formulas do not.

DOI

10.1002/cyto.990100510

Keywords

Bacteria, protist, biomass, digital image analysis

Download

Share Feedback

Included in

Microbiology Commons

Share

COinS