Document Type

Article

Department/Program

Virginia Institute of Marine Science

Publication Date

1997

Journal

Journal of Applied Microbiology

Volume

83

Issue

1

First Page

120

Last Page

126

Abstract

A modified mE medium (mEI) containing the chromogenic substrate indoxyl-beta-D-glucoside to detect beta-D-glucosidase activity was evaluated with respect to specificity and recovery of enterococci from environmental eaters. Extending incubation from 24 to 48 h improved enterococci recovery but 77% of the colonies classified as non-target were confirmed as enterococci. Randomly chosen enterococcal isolates from sewage, exposed in microcosms containing 0.22 mu m membrane filtered fresh or estuarine water, exhibited differences in persistence as a function of exposure treatment. Decreasing the concentration of or eliminating indoxyl-beta-D-glucoside from mE did not significantly affect recovery of purified isolates.

DOI

10.1046/j.1365-2672.1997.00200.x

Download

Share Feedback

Share

COinS