Document Type
Article
Department/Program
Virginia Institute of Marine Science
Publication Date
1997
Journal
Parasite-Journal De La Societe Francaise De Parasitologie
Volume
4
Issue
1
First Page
67
Last Page
73
Abstract
In the present study we describe a protein-free, chemically defined culture medium, designated JL-ODRP-3, which supports the propagation of Perkinsus marinus, a parasite of the eastern oyster, Crassotrea virginica. P. marinus adapted rapidly to the defined medium and the growth rate of the protozoan increased significantly following a few subcultures. Two isolates of P. marinus, one from the Chesapeake Bay (Virginia) and the other from the Gulf of Mexico (Texas) were cultured for at least ten passes. The doubling times far the isolates from Virginia and Texas, in Jog phase, were 18 +/- 1.2 and 28.6 +/- 3.2 hours respectively, after ten passes in JL-ODRP-3. Moreover, P. marinus cells cultured in the defined medium were infective to eastern oysters. Finally, the defined medium was used successfully to initiate continuous cultures of P. marinus from heart fragments of infected oysters. The absence of proteins and peptides from this chemically defined medium demarcates JL-ODRP-3 as the most suitable medium to study P. marinus proteins, to produce antigens far antibody production, and to screen chemotherapeutic agents.
DOI
10.1051/parasite/1997041067
Keywords
Perkinsus marinus; protozoan; in vitro; protein-free chemically defined culture medium; oyster parasite; Crassostrea virginica
Recommended Citation
LaPeyre, JF and Faisal, M, Development of a protein-free chemically defined culture medium for the propagation of the oyster pathogen Perkinsus marinus (1997). Parasite-Journal De La Societe Francaise De Parasitologie, 4(1), 67-73.
10.1051/parasite/1997041067
